dapi nuclear stain (blue) secondary  (Thermo Fisher)

Journal: Investigative Ophthalmology & Visual Science

Article Title: Oxytocin Expression and Function in the Posterior Retina: A Novel Signaling Pathway

doi: 10.1167/iovs.14-15646

Figure Lengend Snippet: Antibodies Used in Immunohistochemistry (IHC) and Western Analysis

Article Snippet: The antibodies used in this study are listed in . table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Source Anti–oxytocin receptor (OXTR) Primary rabbit polyclonal Novus Biologicals, Littleton, CO, USA 1:300 (IHC) 1:1,000 (Western) Anti–oxytocin receptor (OXTR) Primary mouse monoclonal R&D Systems, Minneapolis, MN, USA 1:1,000 (Western) Anti-oxytocin (OXT) Primary rabbit polyclonal Abcam, Cambridge, MA, USA 1:300 (IHC) Anti-RPE65 Primary mouse monoclonal Abcam 1:300 (IHC) Anti–cone arrestin 7G6 (7G6) Primary mouse monoclonal Gift from Peter MacLeish, Atlanta, GA, USA 1:300 (IHC) Lectin PNA Alexa Fluor 594 conjugate Life Technologies, Grand Island, NY, USA Anti–β-actin (β-actin) Primary mouse monoclonal LI-COR Biosciences, Lincoln, NE, USA 1:1,000 (Western) Anti-GAPDH 14C10 Primary rabbit monoclonal Cell Signaling, Boston, MA, USA 1:1,000 (Western) Anti-mouse Alexa-Fluor 488 (green) Secondary goat Invitrogen, Grand Island, NY, USA 1:1,000 (IHC) Anti-rabbit Alexa-Fluor 594 (red) Secondary goat Invitrogen 1:1,000 (IHC) DAPI nuclear stain (blue) Secondary Molecular Probes, Inc., Eugene, OR, USA 1:1,000 (IHC) Anti-mouse IR dye 680RD Secondary LI-COR Biosciences 1:15,000 (Western) Anti-rabbit IR dye 800CW Secondary LI-COR Biosciences 1:15,000 (Western) Open in a separate window Antibodies Used in Immunohistochemistry (IHC) and Western Analysis Tissue Preparation for Immunohistochemistry Rhesus eyes were opened at the pars plana, immersion fixed in 4% paraformaldehyde for 15 minutes, and cryopreserved using a 5%, 10%, and 20% gradient of ice-cold sucrose for 24 hours at each concentration.

Techniques: Immunohistochemistry, Western Blot, Staining